Integrating an algal β-carotene hydroxylase gene into a designed carotenoid-biosynthesis pathway increases carotenoid production in yeast. (May 2015)

• Record Type: Journal Article
• Authors: Chang, Jui-Jen; Thia, Caroline; Lin, Hao-Yeh; Liu, Hsien-Lin; Ho, Feng-Ju; Wu, Jiunn-Tzong; Shih, Ming-Che; Li, Wen-Hsiung; Huang, Chieh-Chen
• Is Part Of: Bioresource technology. Volume 184(2015)
• Page Start: 2
• Page End: 8
• Publication Date: 2015-05
• Subjects: Green algae -- Carotenoids -- β-Carotene hydroxylase -- Synthetic biology; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2014.11.097
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Carotenoid biosynthesis genes from green algae. A designed carotenoid biosynthesis pathway in yeast. Bioengineering approaches to reducing manufacturing cost. Abstract: The algal β-carotene hydroxylase gene Crchyb from Chlamydomonas reinhardtii, Czchyb from Chlorella zofingiensis, or Hpchyb from Haematococcus pluvialis and six other carotenoid-synthesis pathway genes were co-integrated into the genome of a yeast host. Each of these three algal genes showed a higher efficiency to convert β-carotene to downstream carotenoids than the fungal genes from Phaffia rhodozyma . Furthermore, the strain with Hpchyb displayed a higher carotenoid productivity than the strains integrated with Crchyb or Czchyb, indicating that Hpchyb is more efficient than Crchyb and Czchyb . These results suggest that β-carotene hydroxylase plays a crucial role in the biosynthesis of carotenoids.