Screening and characterization of oleaginous Chlorella strains and exploration of photoautotrophic Chlorella protothecoides for oil production. (May 2015)

• Record Type: Journal Article
• Authors: Sun, Zheng; Zhou, Zhi-gang; Gerken, Henri; Chen, Feng; Liu, Jin
• Is Part Of: Bioresource technology. Volume 184(2015)
• Page Start: 53
• Page End: 62
• Publication Date: 2015-05
• Subjects: Algae -- Biofuels -- Chlorella -- Lipid -- Outdoor photobioreactor; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2014.09.054
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: The growth and oil production of nine Chlorella strains were compared. Culture conditions were optimized for oil production by C. protothecoides CS-41. Outdoor oil production by C. protothecoides CS-41 was assessed in a panel PBR. A two-stage culture strategy was proposed for oil production in PBRs. High oil content (55% of dry weight) and productivity (0.59 g L −1 d −1 ) were obtained. Abstract: The growth and oil production of nine Chlorella strains were comparatively assessed and C hlorella protothecoides CS-41 demonstrated the greatest lipid production potential. The effects of different nitrogen forms and concentrations, phosphorus concentrations and light intensities on growth and oil production were studied in laboratory columns. C. protothecoides CS-41 accumulated lipids up to 55% of dry weight, with triacylglycerol and oleic acid being 71% of total lipids and 59% of total fatty acids, respectively. High biomass and lipid productivities were achieved in outdoor panel PBRs, up to 1.25 and 0.59 g L −1 day −1, or 44. 1 and 16.1 g m −2 day −1, respectively. A two-stage cultivation strategy was proposed to enhance the algal biomass and lipid production. This is the first comprehensive investigation of both indoor and outdoor photoautotrophic C. protothecoides cultures for oil production, and C. protothecoides CS-41 represents a promising biofuel feedstock worthy of further exploration.