Bioconversion of lignocellulosic pretreatment effluent via oleaginous Rhodococcus opacus DSM 1069. (January 2015) - Wells, Tyrone
Abstract: Rhodococcus opacus DSM 1069 utilized pine organosolv pretreatment effluent as a sole carbon and energy source for 120 h at 1.5 w/v% solids concentration and accumulated a maximum of 26.99 ± 2.88% of its cellular dry weight in oils composed of oleic, palmitic, and stearic fatty acids. These results establish the potential for lignocellulosic pretreatment effluent as a feedstock for microbial biodiesel production via oleaginous R. opacus and an interesting route for biorefinery waste stream optimization. Highlights: Bacteria converted biorefinery pretreatment waste into fats for biofuel production. Waste streams contained degraded sugars and aromatics. Oleaginicity up to 26.88% of the cellular dry weight was displayed after 48 h. Results establish novel biodiesel production and waste treatment route.