Cultivation of the benthic microalga Prorocentrum lima for the production of diarrhetic shellfish poisoning toxins in a vertical flat photobioreactor. (March 2015)

• Record Type: Journal Article
• Authors: Wang, Shuai; Chen, Junhui; Li, Zhaoyong; Wang, Yanlong; Fu, Boqiang; Han, Xiaotian; Zheng, Li
• Is Part Of: Bioresource technology. Volume 179(2015)
• Page Start: 243
• Page End: 248
• Publication Date: 2015-03
• Subjects: DSP diarrhetic shellfish poisoning -- OA okadaic acid -- DTX1 dinophysistoxin-1 -- SWDSC step-wise double-sedimentation centrifugation; Benthic microalgal cultivation -- Okadaic acid -- Dinophysistoxin-1 -- Photobioreactor -- Microalgal harvesting; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2014.12.019
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: The cultivation conditions for DSP toxin-containing Prorocentrum lima were optimized. A method for large-volume cultivation of P. lima using photobioreactor was developed. P. lima exhibits good growth performance and toxin production in the photobioreactor. A SWDSC method was proposed and used to harvest of the benthic microalgae cells. The total contents of the DSP toxins accounted for 3.6% of the dry microalgal powder. Abstract: In this study, the cultivation conditions of Prorocentrum lima, including temperature, nutrient concentration, photoperiod, and salinity were observed, and then an effective method for the large-volume cultivation of P. lima using a vertical flat photobioreactor was developed for the first time. The maximum cell concentrations and toxin contents of P. lima cultured in the photobioreactor were reached after a 35 days cultivation. Moreover, a step-wise double-sedimentation centrifugation method was used to harvest the microalgae cells, with the harvest rate of 89%. Toxin analysis of dry microalgal powder indicated that OA and DTX1 contents were 15.2 and 21.6 mg g −1, respectively. These results verify that the culture method using the proposed photobioreactor is effective to massively produce DSP toxin-containing P. lima . This study may serve as a guide for the large-scale production of toxin-producing red-tide benthic microalgae.