Effect of inulin on efficient production and regulatory biosynthesis of bacillomycin D in Bacillus subtilis fmbJ. (March 2015)

• Record Type: Journal Article
• Authors: Qian, Shiquan; Lu, Hedong; Meng, Panpan; Zhang, Chong; Lv, Fengxia; Bie, Xiaomei; Lu, Zhaoxin
• Is Part Of: Bioresource technology. Volume 179(2015)
• Page Start: 260
• Page End: 267
• Publication Date: 2015-03
• Subjects: ATP adenosine triphosphate -- BEM beef extract medium -- BYA bmyA -- BYB bmyB -- BYC bmyC -- DTNB 5, 5′-dithio-bis(2-nitrobenzoic acid) -- HEPES N-(2-hydroxyethyl)piperazine-N′-2-ethanesulfonate -- HPLC–MS/MS high performance liquid chromatography–mass spectrometry/mass spectrometry -- LM0 a modified Landy medium -- NRPSs non-ribosomal peptide synthetases -- PBS phosphate buffered saline -- RP-HPLC reverse-phase high performance liquid chromatography -- RT-qPCR quantitative real-time reverse-transcriptase polymerase chain reaction -- TE thioesterase gene; Inulin -- Bacillomycin D production -- Fed-batch fermentation -- Gene expression -- Regulatory mechanism; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2014.11.086
• Languages: English
• ISSNs: 0960-8524

Abstract

Abstract : Graphical abstract: Highlights: Inulin proved to be efficient in enhancing bacillomycin D biosynthesis. Fed-batch fermentation greatly improved the bacillomycin D production. Inulin promoted the expression of the bacillomycin D synthetase genes. Up-regulation of signal proteins heightened the bacillomycin D production. Abstract: The effect of inulin on the production of bacillomycin D and the levels of mRNA of bacillomycin D synthetase genes: bmyA ( BYA ), bmyB ( BYB ), bmyC ( BYC ), the thioesterase gene ( TE ) and regulating genes: AbrB, ComA, DegU, PhrC, SigmaH and Spo0A in Bacillus subtilis fmbJ were investigated. The production of bacillomycin D was enhanced with the increase of biomass concentration. The maximum production and productivity of bacillomycin D were found to be 1227.49 mg/L and 10.23 mg/L h. Inulin significantly improved the expression of bacillomycin D synthetase genes: bmyA ( BYA ), bmyB ( BYB ), bmyC ( BYC ) and the thioesterase gene ( TE ). Also, inulin up-regulated ComA, DegU, SigmaH and Spo0A and therefore promoted the high production of bacillomycin D. Our results provided a practical approach for efficient production of bacillomycin D and a meaningful explanation for regulatory mechanism of bacillomycin D biosynthesis.