Peroxynitrite decomposition catalyst prevents matrix metalloproteinase-9 activation and neurovascular injury after hemoglobin injection into the caudate nucleus of rats. (25th June 2015)

• Record Type: Journal Article
• Authors: Ding, R.; Feng, L.; He, L.; Chen, Y.; Wen, P.; Fu, Z.; Lin, C.; Yang, S.; Deng, X.; Zeng, J.; Sun, G.
• Is Part Of: Neuroscience. Volume 297(2015)
• Page Start: 182
• Page End: 193
• Publication Date: 2015-06-25
• Subjects: ICH intracerebral hemorrhage -- Hb hemoglobin -- BBB blood–brain barrier -- ONOO− peroxynitrite -- 3-NT 3-nitrotyrosine -- CNS central nervous system -- nNOS neuronal nitric oxide synthase -- eNOS endothelial nitric oxide synthase -- iNOS inducible nitric oxide synthase -- IR ischemia reperfusion -- MCAO middle cerebral artery occlusion -- mNSS modified neurological severity score -- NO nitric oxide -- NOS nitric oxide synthase -- RNS reactive nitrogen species -- ROS reactive oxygen species -- Sham sham-operated animals -- NF-κB nuclear factor-кB -- ZO-1 zonula occludens-1; peroxynitrite -- matrix metalloproteinases -- neurovascular injury -- hemoglobin -- intracerebral hemorrhage -- oxidative or nitrative stress; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2015.03.065
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Hb-induced ONOO − formation may contribute to neurovascular dysfunction and subsequent apoptosis after ICH. ONOO − production may be involved in the NF-κB induction and MMP-9 activation after ICH. FeTPPS treatment could partly ameliorate the destructive role of Hb after ICH. Reducing ONOO − accumulation, thereby inhibiting MMP-9 activation, could be a new therapeutic target for ICH. Abstract: Hemoglobin (Hb) is a major constituent of blood and a potent mediator of oxidative or nitrative stress after intracerebral hemorrhage (ICH). Our previous study demonstrated that Hb could induce abundant peroxynitrite (ONOO − ) formation in vivo, which may be involved in the blood–brain barrier (BBB) disruption, however, the drug intervention is absent and also the underlying mechanism. Using an experimental stroke model by injecting Hb into the caudate nucleus of male Sprague–Dawley rats, we assessed the role of ONOO − decomposition catalyst, 5, 10, 15, 20-tetrakis (4-sulfonatophenyl) porphyrinato iron(III) [FeTPPS] in the activation of MMP-9 and Hb-induced neurovascular injuries. 3-Nitrotyrosine (3-NT, as an index of ONOO − formation) and NF-κB expression was measured by western blot (WB) and immunohistochemistry (IHC)/immunofluorescence (IF). Activity of MMP was evaluated by in situ zymography. Neurovascular injury was assessed using zonula occludens-1 (ZO-1) by WB and IF, fibronectin (FN) and neuron-specific nuclear protein (NeuN) IHC. Perihematomal cell death was determined by TUNEL assay. Behavioral outcome was measured by modified neurological severity score (mNSS) test. At the injured striata, profuse 3-NT was produced and mainly expressed in neutrophils and microglia/macrophages. 3-NT formation significantly colocalized with nuclear factor-κB (NF-κB) expression. In situ zymography showed that gelatinase activity was mostly co-localized with neurons and blood vessel walls and partly with neutrophils and microglia/macrophages. Enhanced 3-NT production, NF-κB induction and MMP-9 activation were obviously reduced after FeTPPS treatment. Hb-induced injury to tight junction protein (ZO-1), basal lamina of FN-immunopositive microvasculature and neural cells was evidently ameliorated by FeTPPS. In addition, apoptotic cell numbers as well as behavioral deficits were also improved. The present study shows that the administration of the ONOO − decomposition catalyst FeTPPS protects against Hb-induced neurovascular injuries and improves neurological function, which possibly in part by suppressing MMP-9 activation.