Deoxynivalenol impairs proliferation and induces apoptosis in primary murine osteoblasts. Issue 2 (7th February 2018)

• Record Type: Journal Article
• Authors: Wu, Huihui; Sun, Xiao; Zhang, Zhaoqiang; Zhuang, Dongming; Wang, Yu; Li, Xiaoxia; Yu, Guangfu; Lv, Gang; Wang, Na; Li, Qiaoling; Wang, Charles Howard; Yu, Ailian; Zhao, Yinghui
• Is Part Of: Toxicological and environmental chemistry. Volume 100:Issue 2(2018)
• Page Start: 214
• Page End: 227
• Publication Date: 2018-02-07
• Subjects: Deoxynivalenol -- osteoblasts -- apoptosis -- Bcl-2-associated X protein -- B-cell lymphoma-2; Toxicology -- Periodicals; Environmental chemistry -- Periodicals; 571.95
• DOI: 10.1080/02772248.2018.1458851
• Languages: English
• ISSNs: 0277-2248

Abstract

ABSTRACT: The effects of deoxynivalenol in different dose including 100, 200, 500, 1000, and 1500 µg/L on primary cranial osteoblasts from fetal mice were investigated. Fluorescence staining, flow cytometric measurement, 3-(4, 5-dimethylthiazol 2-y1)-3, 5-di-phenyltetrazolium bromide assay, quantitative PCR, and Western blot were used for the test. Mineralization and proliferation of osteoblasts decreased upon 100 µg/L and higher deoxynivalenol treatment and apoptosis of osteoblasts was increased upon 500, 1000, and 1500 µg/L deoxynivalenol treatment. Karyopyknosis, membrane breakage, and a decreased number of calcium nodes were also observed upon 500 µg/L deoxynivalenol treatment. The mRNA and protein levels of B-cell lymphoma-2-associated X protein were upregulated, B-cell lymphoma-2 protein downregulated with increasing concentrations of deoxynivalenol treatment and their ratio increased. Deoxynivalenol induces apoptosis of osteoblasts, suggesting a mechanism by which deoxynivalenol can affect murine skeletal development.