Epigenetic effect of long-term bisphenol A exposure on human breast adenocarcinoma cells. Issue 2 (7th February 2018)

• Record Type: Journal Article
• Authors: Wang, Xiaoli; Wei, Yunbo; Guo, Tao; Yuan, Jinpeng; Zhao, Rusong
• Is Part Of: Toxicological and environmental chemistry. Volume 100:Issue 2(2018)
• Page Start: 258
• Page End: 266
• Publication Date: 2018-02-07
• Subjects: DNA methylation -- bisphenol A -- human breast cancer cell -- epigenotoxicity; Toxicology -- Periodicals; Environmental chemistry -- Periodicals; 571.95
• DOI: 10.1080/02772248.2018.1466143
• Languages: English
• ISSNs: 0277-2248

Abstract

ABSTRACT: In this research, epigenetic effects of bisphenol A (BPA) on human breast cancer MCF-7 cells were analyzed. Genome-wide DNA methylation and gene expression were analyzed in MCF-7 cells exposed to BPA (10 −5 and 10 −6 mol/L for 5 weeks). No significant changes in the global level of 5-methyl-2′-deoxycytidine and 5-hydroxymethyl-2′-deoxycytidine were observed. DNA methylation profiling analysis indicated that BPA exposure resulted in the hypermethylation of FOXK2, LKB1, LMX1A and CUGBP2 and the hypomethylation of PTPRN2, TRIM27, BCAS3 and ZNF423 . Decreased expression of apoptosis genes ( P38 and BCL2L1 ) and increased expression of chemokine ( Cxcl2 and ccl20 ) were detected. Changes of these genes were speculated to affect the ERα-related cell growth as well as cell apoptosis.