Determining Symbiotic Nitrogen Fixation in Dry Bean Cultivars Using Ureide Method and Isotope Dilution Techniques. Issue 16 (8th September 2018)

• Record Type: Journal Article
• Authors: Sanyal, Debankur; Goos, Robert Jay; Chatterjee, Amitava
• Is Part Of: Communications in soil science and plant analysis. Volume 49:Issue 16(2018)
• Page Start: 2042
• Page End: 2052
• Publication Date: 2018-09-08
• Subjects: 15N -- dry bean -- isotope dilution -- Rhizobium -- symbiotic nitrogen fixation -- ureide; Soil science -- Periodicals; Plants -- Chemical analysis -- Periodicals; Agricultural chemistry -- Periodicals; 631.405
• DOI: 10.1080/00103624.2018.1495727
• Languages: English
• ISSNs: 0010-3624

Abstract

ABSTRACT: Symbiotic nitrogen fixation (SNF) is an environmentally safe source of nitrogen (N) to the crop plants. In total, 12 dry bean ( Phaseolus vulgaris L.) cultivars from pinto, navy, black, and kidney market classes were inoculated with rhizobia and grown in a greenhouse. SNF was estimated using isotope dilution technique and 'ureide' method. The amount of SNF ranged between 33 and 68 mg N plant –1 when determined using 15 N isotope dilution and followed the order: pinto > navy > black > kidney. Percent N derived from atmosphere (%Ndfa) significantly varied between 49% and 90% at V3 and between 71% and 98% at R2 stages. The outcomes of the experiment suggested that dry bean cultivars from different market classes have variable N2 fixation ability, and fertilizer N required should be calculated according to their SNF potentials and N need of a specific market class or cultivar. Stable isotope dilution should be used as the standard procedure to estimate the SNF in dry bean.