Small interfering RNA based knock down of acute heat shock and or GGA inducible bovine heat shock protein 70 may interfere invitro expression pattern of TLR2/4 and NOD1/2. (October 2018)

• Record Type: Journal Article
• Authors: Sengar, Gyanendra Singh; Kant, Rajiv; Deb, Rajib; Verma, Preetam
• Is Part Of: Journal of thermal biology. Volume 77(2018)
• Page Start: 75
• Page End: 85
• Publication Date: 2018-10
• Subjects: Hsp70 -- PRRs -- TLR2/4 -- NOD1/2 -- siRNA -- GGA; Thermobiology -- Periodicals; Temperature -- Periodicals; Biology -- Periodicals; Thermobiologie -- Périodiques; Thermobiology; Periodicals; 571.46
• DOI: 10.1016/j.jtherbio.2018.08.015
• Languages: English
• ISSNs: 0306-4565

Abstract

Abstract: Heat shock protein 70 (Hsp70) is a widely known mammalian stress-inducible protein critical for cytoprotection against different stressors. Despite the chaperone based cytoprotective functions, intracellular Hsp70 also participates in diverse immune modulatory activities. To investigate the immune modulatory effect of inducible bovine Hsp70, we examined the expression profile of four major pattern recognition receptors (PRRs) viz. TLR2/4 and NOD1/2 using two different invitro cell cultured models. We observed that, invitro acute heat stress and Geranylgeranylacetone (GGA) induction increased the level of Hsp70 which upregulated the expression of all the four PRRs in both the cell cultured models. However, the expression level of TLR4 was found to be highest followed by NOD2, TLR2 and NOD1. Conversely, specific siRNA based knockdown of Hsp70 showed a decreased expression level of all the four PRRs. This study may add some references pertaining to the innate immune modulatory effects of bovine heat shock protein 70. Highlights: Effect of GGA (HSP70 Inducer) was evaluated in Bovine PBMC and MDBK cell line. siRNAs based knockdown of bovine HSP70 revealed down regulation of TLR2/4 and NOD1/2. Results indicated that HSP70 plays important role in the expression profiling of TLR2/4 and NOD1/2.