Electrochemical investigation of an interaction of the antidepressant drug aripiprazole with original and damaged calf thymus dsDNA. (1st July 2015)

• Record Type: Journal Article
• Authors: Kurbanoglu, Sevinc; Dogan-Topal, Burcu; Hlavata, Lenka; Labuda, Jan; Ozkan, Sibel A.; Uslu, Bengi
• Is Part Of: Electrochimica acta. Volume 169(2015)
• Page Start: 233
• Page End: 240
• Publication Date: 2015-07-01
• Subjects: Aripiprazole -- calf thymus double stranded deoxyribonucleic acid -- voltammetry -- glassy carbon electrode -- DNA biosensor; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2015.04.087
• Languages: English
• ISSNs: 0013-4686

Abstract

Abstract: Interaction of an antipsychotic agent, aripiprazole, with calf thymus double stranded deoxyribonucleic acid, ct-dsDNA, was investigated by differential pulse voltammetry (using two methods; interaction on the dsDNA modified electrode surface and in solution phase) and UV–VIS spectrophotometry. The binding constant between dsDNA and aripiprazole, K, about 3 × 10 5 M −1 was obtained spectrophotometrically in 0.1 M acetate buffer, at pH 4.7. Moreover, the DNA-drug association interaction was confirmed by the differential pulse (DP) voltammetric and spectrophotometric investigations of the systems aripiprazole – polyGuanine (polyG) and aripiprazole – polyAdenine (polyA) in solution phase. The interaction between aripiprazole and damaged ct-dsDNA was also investigated using differential pulse voltammetry.