Modulating effect of d‐carvone on 1, 2‐dimethylhydrazine‐induced pre‐neoplastic lesions, oxidative stress and biotransforming enzymes, in an experimental model of rat colon carcinogenesis. (30th September 2013)

• Record Type: Journal Article
• Authors: Vinothkumar, R.; Sudha, M.; Viswanathan, P.; Kabalimoorthy, J.; Balasubramanian, T.; Nalini, N.
• Is Part Of: Cell proliferation. Volume 46:Number 6(2013:Dec.)
• Page Start: 705
• Page End: 720
• Publication Date: 2013-09-30
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12062
• Languages: English
• ISSNs: 0960-7722

Abstract

Abstract: Objectives: The present study has aimed to evaluate chemopreventive potential ofd ‐carvone on oxidative stress markers, biotransforming enzymes, incidence of colonic polyps and aberrant crypt foci (ACF) in 1, 2‐dimethylhydrazine (DMH)‐induced experimental colon carcinogenesis. Materials and Methods: Rats were randomly divided into six groups, with group I serving as control. Group II animals receivedd ‐carvone every day orally (20 mg/kg body weight) for 16 weeks; groups III–VI received subcutaneous injections of DMH (20 mg/kg body weight) once a week, for the first 4 weeks. In addition, groups IV–VI received different doses ofd ‐carvone (5, 10 and 20 mg/kg body weight everyday orally) along with DMH injections. Results: Our results revealed that supplementation withd ‐carvone significantly reduced incidence of polyps/ACF and ACF multiplicity in DMH‐exposed rats compared to DMH‐alone‐exposed rats. Moreover, our results showed reduced activities of liver and circulatory antioxidants and increased levels of lipid peroxidation by products in DMH‐exposed animals, which were significantly reversed on supplementation withd ‐carvone. In addition, colonic antioxidants and lipid peroxidation were significantly diminished in DMH‐exposed rats, which were significantly elevated on supplementation withd ‐carvone. Furthermore, we also determined activities of biotransforming enzymes, which were found to be altered in DMH‐exposed rats, but reversed ond ‐carvone supplementation. All these observations of changes were supported by histochemical findings. Conclusion: Overall, results obtained from this study suggest thatd ‐carvone at 10 mg/kg body weight provided optimum protection and could be used as an effective chemopreventive agent against colon carcinogenesis induced by DMH.