Responses of plasmid-mediated quinolone resistance genes and bacterial taxa to (fluoro)quinolones-containing manure in arable soil. (January 2015)

• Record Type: Journal Article
• Authors: Xiong, Wenguang; Sun, Yongxue; Ding, Xueyao; Zhang, Yiming; Zhong, Xiaoxia; Liang, Wenfei; Zeng, Zhenling
• Is Part Of: Chemosphere. Volume 119(2015)
• Page Start: 473
• Page End: 478
• Publication Date: 2015-01
• Subjects: Plasmid-mediated quinolone resistance genes -- (Fluoro)quinolones -- Bacterial community -- Manure application; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2014.07.040
• Languages: English
• ISSNs: 0045-6535

Abstract

Highlights: This is the first report to quantify the dissipation rates of PMQR genes. This study first investigates the response of bacterial taxa to FQNs. Significant effects of PMQR genes disappeared 2 month after manure application. FQNs introduced by manure slowed down the dissipation of PMQR genes. FQNs in manure provided a selective advantage for specific bacterial phyla. Abstract: The aim of the present study was to investigate the fate of plasmid-mediated quinolone resistance (PMQR) genes and the disturbance of soil bacterial communities posed by (fluoro)quinolones (FQNs)-containing manure in arable soil. Representative FQNs (enrofloxacin (ENR), ciprofloxacin (CIP) and norfloxacin (NOR)), PMQR genes ( qepA, oqxA, oqxB, aac(6′)-Ib-cr and qnrS ) and bacterial communities in untreated soil, +manure and +manure+FQNs groups were analyzed using culture independent methods. The significantly higher abundance of oqxA, oqxB and aac(6′)-Ib-cr, and significantly higher abundance of qnrS in +manure group than those in untreated soil disappeared at day 30 and day 60, respectively. All PMQR genes ( oqxA, oqxB, aac(6′)-Ib-cr and qnrS ) dissipated 1.5–1.7 times faster in +manure group than those in +manure+FQNs group. The disturbance of soil bacterial communities posed by FQNs-containing manure was also found. The results indicated that significant effects of PMQR genes ( oqxA, oqxB, aac(6′)-Ib and qnrS ) on arable soils introduced by manure disappeared 2 month after manure application. FQNs introduced by manure slowed down the dissipation of PMQR genes. The presence of high FQNs provided a selective advantage for species affiliated to the phylum including Acidobacteria, Verrucomicrobia and Planctomycetes while suppressing Proteobacteria and Actinobacteria .