Self-assembling multidomain peptides tailor biological responses through biphasic release. (June 2015)

• Record Type: Journal Article
• Authors: Kumar, Vivek A.; Taylor, Nichole L.; Shi, Siyu; Wickremasinghe, Navindee C.; D'Souza, Rena N.; Hartgerink, Jeffrey D.
• Is Part Of: Biomaterials. Volume 52(2015)
• Page Start: 71
• Page End: 78
• Publication Date: 2015-06
• Subjects: Self-assembly -- Multi-domain peptide -- Inflammation -- Macrophage polarization; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2015.01.079
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Delivery of small molecules and drugs to tissues is a mainstay of several tissue engineering strategies. Next generation treatments focused on localized drug delivery offer a more effective means in dealing with refractory healing when compared to systemic approaches. Here we describe a novel multidomain peptide hydrogel that capitalizes on synthetic peptide chemistry, supramolecular self-assembly and cytokine delivery to tailor biological responses. This material is biomimetic, shows shear stress recovery and offers a nanofibrous matrix that sequesters cytokines. The biphasic pattern of cytokine release results in the spatio-temporal activation of THP-1 monocytes and macrophages. Furthermore, macrophage–material interactions are promoted without generation of a proinflammatory environment. Subcutaneous implantation of injectable scaffolds showed a marked increase in macrophage infiltration and polarization dictated by cytokine loading as early as 3 days, with complete scaffold resorption by day 14. Macrophage interaction and response to the peptide composite facilitated the (i) recruitment of monocytes/macrophages, (ii) sustained residence of immune cells until degradation, and (iii) promotion of a pro-resolution M2 environment. Our results suggest the potential use of this injectable cytokine loaded hydrogel scaffold in a variety of tissue engineering applications.