An in vitro dynamic microcosm biofilm model for caries lesion development and antimicrobial dose-response studies. Issue 3 (15th March 2016)

• Record Type: Journal Article
• Authors: Maske, T.T.; Brauner, K.V.; Nakanishi, L.; Arthur, R.A.; van de Sande, F.H.; Cenci, M.S.
• Is Part Of: Biofouling. Volume 32:Issue 3(2016)
• Page Start: 339
• Page End: 348
• Publication Date: 2016-03-15
• Subjects: Biofilm model -- enamel demineralization -- artificial dental caries -- chlorhexidine; Marine fouling organisms -- Periodicals; Materials -- Biodeterioration -- Periodicals; Fouling -- Periodicals; Biodegradation -- Periodicals; Environmental Microbiology -- Periodicals; Water Microbiology -- Periodicals; 576.165
• DOI: 10.1080/08927014.2015.1130824
• Languages: English
• ISSNs: 0892-7014

Abstract

Abstract: Some dynamic biofilm models for dental caries development are limited as they require multiple experiments and do not allow independent biofilm growth units, making them expensive and time-consuming. This study aimed to develop and test an in vitro dynamic microcosm biofilm model for caries lesion development and for dose-response to chlorhexidine. Microcosm biofilms were grown under two different protocols from saliva on bovine enamel discs for up to 21 days. The study outcomes were as follows: the percentage of enamel surface hardness change, integrated hardness loss, and the CFU counts from the biofilms formed. The measured outcomes, mineral loss and CFU counts showed dose-response effects as a result of the treatment with chlorhexidine. Overall, the findings suggest that biofilm growth for seven days with 0.06 ml min −1 salivary flow under exposure to 5% sucrose (3 × daily, 0.25 ml min −1, 6 min) was suitable as a pre-clinical model for enamel demineralization and antimicrobial studies.