Urinary biomarkers of oxidative and nitrosative stress and the risk for incident stroke: A nested case–control study from a community-based cohort. (15th March 2015)

• Record Type: Journal Article
• Authors: Lin, Hung-Ju; Chen, Shu-Ting; Wu, Hon-Yen; Hsu, Hsiu-Ching; Chen, Ming-Fong; Lee, Yuan-Teh; Wu, Kuen-Yuh; Chien, Kuo-Liong
• Is Part Of: International journal of cardiology. Volume 183(2015)
• Page Start: 214
• Page End: 220
• Publication Date: 2015-03-15
• Subjects: Urinary biomarkers -- Oxidative stress -- 8-Iso-prostaglandin F2α -- Stroke -- Nested case–control study; Cardiology -- Periodicals; Electronic journals; 616.12
• DOI: 10.1016/j.ijcard.2015.01.043
• Languages: English
• ISSNs: 0167-5273

Abstract

Abstract: Background: Oxidative and nitrosative stress has suggested to be involved in the pathophysiology of cardiovascular diseases, but has unclear relationship with the risk for incident stroke. Methods: In this nested case–control study, cases consisted of 131 participants who were free of stroke at screening and experienced incident stroke during the follow-up period. Controls were 1:1 frequency-matched for age and sex. Baseline levels of urinary creatinine-indexed biomarkers were measured using liquid chromatography–tandem mass spectrometry, including 8-iso-prostaglandin F2α (8-iso-PGF2α ), 4-hydroxynonenal conjugate with mercapturic acid, 8-hydroxydeoxyguanosine and 8-nitroguanine. Results: The levels of urinary 8-iso-PGF2α in stroke cases were higher than in controls [median (interquartile range), 1.13 (2.23–4.36) μg/g creatinine versus 0.71 (1.34–3.02) μg/g creatinine, p = 0.004]. After adjusting cardiovascular risk factors, the association remained that higher level of urinary 8-iso-PGF2α entailed the greater risk for incident stroke [per 1 standard deviation increase in log-transformed value, adjusted odds ratio, 1.40; 95% confidence interval (CI), 1.06–1.85; p = 0.005] with a significant increasing trend across its quartiles (p for trend = 0.016). After adding urinary 8-iso-PGF2α, the prediction model not only improved discrimination between participants with or without incident stroke (integrated discrimination improvement, 0.025; 95% CI, 0.006–0.045; p = 0.005), but enhanced stroke risk stratification (net reclassification improvement, 19.8%; 95% CI, 4.6–35.1%; p = 0.011). In contrast, the relationships were non-significant among the other three biomarkers. Conclusions: Our findings demonstrated that urinary 8-iso-PGF2α could be an independent biomarker of oxidative stress for prediction of the risk for incident stroke.