Aqueous Two-Phase Flotation for the Recovery of Biomolecules. Issue 1 (2nd January 2016)

• Record Type: Journal Article
• Authors: Lee, Sze Ying; Khoiroh, Ianatul; Ling, Tau Chuan; Show, Pau Loke
• Is Part Of: Separation and purification reviews. Volume 45:Issue 1(2016)
• Page Start: 81
• Page End: 92
• Publication Date: 2016-01-02
• Subjects: Aqueous two-phase flotation -- surface active compounds -- aqueous two-phase system -- solvent sublation; Separation (Technology) -- Periodicals; Chemicals -- Purification -- Periodicals; 660.2842
• DOI: 10.1080/15422119.2015.1007147
• Languages: English
• ISSNs: 1542-2119

Abstract

Abstract : Aqueous two-phase flotation (ATPF), a novel technique which integrates the principles of aqueous two-phase systems and mass transfer mode of solvent sublation, has been successfully applied for the separation and purification of biological materials in the field of biotechnology. From the studies done so far, ATPF has demonstrated as an effective, economical and environment-friendly technique for separation of labile biomolecules from their crude aqueous extracts of natural plants or fermentation broths, under mild separation condition. In this article, the basic principles, experimental apparatus set up, key design variables of ATPF, ATPF applications and a comparative study between ATPF and other conventional methods are discussed. This review is intended to present a comprehensive summary on the research works carried out so far related to ATPF since its introduction in 2009 until present, which will be helpful for further research in ATPF.