Physiological and biochemical response of Aedes aegypti tolerance to Bacillus thuringiensis*. Issue 2 (1st February 2016)

• Record Type: Journal Article
• Authors: Wu, Songqing; Wu, Wei; Zhu, Xiaoli; Liu, Zhaoxia; Rebeca, Carballar-Lejarazú; Fu, Tao; Han, Chaoqiang; Shao, Ensi; Pan, Xiaohong; Huang, Zhipeng; Xu, Lei; Zou, Shuangquan; Zhang, Lingling; Guan, Xiong
• Is Part Of: Biocontrol science and technology. Volume 26:Issue 2(2016)
• Page Start: 227
• Page End: 238
• Publication Date: 2016-02-01
• Subjects: Bacillus thuringiensis -- Aedes aegypti -- detoxifying enzymes -- mosquito tolerance; Pests -- Biological control -- Periodicals; Weeds -- Biological control -- Periodicals; Pesticides -- Environmental aspects -- Periodicals; Plants, Protection of -- Periodicals; Plants -- Biological control -- Periodicals; Agricultural pests -- Control -- Periodicals; Pest Control, Biological -- Periodicals; 632.96
• DOI: 10.1080/09583157.2015.1089216
• Languages: English
• ISSNs: 0958-3157

Abstract

ABSTRACT: Bacillus thuringiensis subsp. israelensis (Bti) represents the only eco-friendly bio-degradable insecticide for mosquito-borne disease control. Our research aims to identify if mosquito detoxification enzymes play an important role in Bti tolerance mechanisms in the dengue vector, Aedes aegypti . Several enzymes, such as amylase, cytochromes P450, Na + /K + -ATPase, acetylcholinesterase, protease and glutathione S -transferase (GST) were analysed and level of activity determined in Ae. aegypti larvae after Bti treatment. Bti exposure significantly increased the level of amylase (183.2%) as well as the activity of cytochromes P450 (177.5%), and Na + /K + -ATPase (142.9%). On the other hand, there was a decrease of 8.6% and 11.4% in acetylcholinesterase and GST activity, and no significant effect in the total level of protease activity. We suggest that the variation in amylase, cytochromes P450, Na + /K + -ATPase, acetylcholinesterase, protease and GST activity may be associated with the Bti insecticidal mechanism. This study provides the basis of detoxifying enzymes in Bti tolerance.