Elevated miR-20b-5p expression in peripheral blood mononuclear cells: A novel, independent molecular biomarker of favorable prognosis in chronic lymphocytic leukemia. (July 2018)

• Record Type: Journal Article
• Authors: Papageorgiou, Sotirios G.; Kontos, Christos K.; Tsiakanikas, Panagiotis; Stavroulaki, Georgia; Bouchla, Anthi; Vasilatou, Diamantina; Bazani, Efthymia; Lazarakou, Afroditi; Scorilas, Andreas; Pappa, Vasiliki
• Is Part Of: Leukemia research. Volume 70(2018)
• Page Start: 1
• Page End: 7
• Publication Date: 2018-07
• Subjects: AUC area under the curve -- BCa bias corrected and accelerated -- CI confidence interval -- CLL chronic lymphocytic leukemia -- DLBCL diffuse large B-cell lymphoma -- HIF1A hypoxia-inducible factor 1 alpha subunit -- IGHV immunoglobulin heavy chain variable region -- miR-20b-5p microRNA-20b-5p -- miRNA microRNA -- mRNA messenger RNA -- OR odds ratio -- OS overall survival -- PBMC peripheral blood mononuclear cell -- PTEN phosphatase and tensin homolog -- qPCR quantitative real-time PCR -- ROC receiver operating characteristic -- RQU relative quantification unit -- SNORD43 small nucleolar RNA C/D box43 -- ZAP70 zeta-chain–associated protein kinase 70 kDa; microRNA -- Prognostic biomarker -- Favorable prognosis -- CLL -- Hematological malignancy -- Tumor marker; Leukemia -- Periodicals; Leukemia -- Periodicals; Leucémie -- Périodiques; Leukemia; Periodicals; Electronic journals; Electronic journals; 616.9941905
• DOI: 10.1016/j.leukres.2018.04.014
• Languages: English
• ISSNs: 0145-2126

Abstract

Highlights: A cost-effective qPCR method for quantification of miR-20b-5p levels was developed. Low miR-20b-5p expression predicts poor overall survival in CLL patients. Prognostic significance of miR-20b-5p is independent of clinicopathological factors. miR-20b-5p is a favorable prognosticator in distinct subgroups of CLL patients. Abstract: MicroRNA-20b-5p (miR-20b-5p) is part of the miR-106a/363 cluster and a member of the cancer-related miR-17 family. miR-20b-5p regulates important transcription factors, including hypoxia-inducible factor 1 (HIF1) and signal transducer and activator of transcription 3 (STAT3). Recently, the dysregulation of miR-20b-5p expression has been observed in many B-cell lymphomas and T-cell leukemias. In this research study, we examined the putative prognostic value of miR-20b-5p in CLL. Therefore, total RNA was isolated from peripheral blood mononuclear cells (PBMCs) collected from 88 CLL patients; next, total RNA was polyadenylated and first-strand cDNA was synthesized, using an oligo-dT–adapter primer. miR-20b-5p expression was quantified using an in-house–developed real-time quantitative PCR assay. Kaplan-Meier OS analysis and bootstrap univariate Cox regression showed that high miR-20b-5p expression predicts better OS for CLL patients ( p < 0.001). Interestingly, miR-20b-5p overexpression retains its favorable prognostic role in CLL patients of intermediate risk or stratified according to established prognostic factors [CD38 expression and mutational status of the immunoglobulin heavy chain variable ( IGHV ) region]. In conclusion, miR-20b-5p is a potential independent molecular biomarker of favorable prognosis in CLL.