Single-walled carbon nanotubes affect the expression of genes associated with immune response in normal human astrocytes. (October 2018)

• Record Type: Journal Article
• Authors: Minchenko, Oleksandr H.; Tsymbal, Dariia O.; Minchenko, Dmytro O.; Prylutska, Svitlana V.; Hnatiuk, Oksana S.; Prylutskyy, Yuriy I.; Tsierkezos, Nikos G.; Ritter, Uwe
• Is Part Of: Toxicology in vitro. Volume 52(2018)
• Page Start: 122
• Page End: 130
• Publication Date: 2018-10
• Subjects: Single-walled carbon nanotubes -- HLA-DRA expression -- Normal human astrocytes; HLA-DRA major histocompatibility complex, class II, DR alpha -- HLA-DRB1 major histocompatibility complex, class II, DR beta 1 -- HLA-F major histocompatibility complex, class I, F -- LMNB1 lamin B1 -- HTRA1 high-temperature requirement A serine peptidase 1 -- PHLDA2 pleckstrin homology-like domain, family A, member 2 -- PLOD2 procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 -- SEM scanning electron microscopy -- SWCNTs single-walled carbon nanotubes -- TEM transmission electron microscopy; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2018.06.011
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: The effect of single-walled carbon nanotubes (SWCNTs) on the expression of a subset of immune response, apoptosis and cell proliferation –associated genes was studied in normal human astrocytes (line NHA/TS). In the cells treated with SWCNTs (2, 10 and 50 ng/ml of medium for 24 h) we observed a strong dose-dependent down-regulation of the expression of a cell surface glycoproteins HLA-DRA (major histocompatibility complex, class II, DR alpha) and HLA-DRB1. At the same time, the expression of HLA-F (major histocompatibility complex, class I, F), LMNB1 (lamin B1), and HTRA1 (high temperature requirement A1) genes as well as the level of miR-190b and miR-7 was up-regulated in NHA/TS subjected to different concentrations of SWCNTs. After 24 h of treatment with SWCNTs we detected a dose-dependent suppression of PHLDA2 (pleckstrin homology-like domain, family A, member 2) gene expression in these cells. Obtained data show that SWCNTs may affect an immune response, in particular through suppression of HLA-DRA and HLA-DRB1 gene expressions and that miR-190b and miR-7 possibly participated in this suppression. Deregulation of lamin B1 expression indicates the possibility of alterations in genome stability following treatment of astrocytes with SWCNTs. Thus, more caution is needed in biomedical application of SWCNTs. Highlights: SWCNTs affect immune response through suppression of cell surface glycoproteins HLA-DR MicroRNA miR-7 and miR-190b are upregulated by SWCNTs and suppress immune response Deregulation of lamin B1 expression indicates genome stability alterations in cells treated by SWCNTs