Antimicrobial and cytotoxic effects of denture base acrylic resin impregnated with cleaning agents after long-term immersion. (October 2018)

• Record Type: Journal Article
• Authors: Procópio, A.L.F.; da Silva, R.A.; Maciel, J.G.; Sugio, C.Y.C.; Soares, S.; Urban, V.M.; Neppelenbroek, K.H.
• Is Part Of: Toxicology in vitro. Volume 52(2018)
• Page Start: 8
• Page End: 13
• Publication Date: 2018-10
• Subjects: Denture acrylic resin -- Chemical denture cleansers -- Long-term immersion -- Chemical residues -- Antimicrobial effect -- Cytotoxicity; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2018.05.012
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: The coadjutant method for denture cleansing most used by denture wearers is immersion in chemical agents, which are toxic when in direct contact with cells. However, clinically, the contact between these chemical agents and prosthetic tissues does not occur directly, but rather with what remained impregnated into acrylic bases, even after rinsing the disinfected dentures. This study evaluated the antimicrobial and cytotoxic effects of a denture acrylic resin after successive cycles of daily overnight immersion in 1% sodium hypochlorite (1%NaClO) and 2% chlorhexidine digluconate (2%CHX), simulating the periods of 9 months or 1.5 year. Microbiological and cytotoxic assays were performed, respectively, by broth microdilution method ( Candida albicans or Staphylococcus aureus ) and MTT assay. Chemical residues of 2%CHX impregnated into the denture acrylic resin had an antimicrobial effect on both immersion periods, which was not observed with those of 1%NaClO. However, residues of 2%CHX were severely cytotoxic to human gingival fibroblasts compared to those of 1%NaClO and acrylic resin (not submitted to the denture cleansers), which were slightly cytotoxic. Even at low concentrations recommended for overnight soaking of removable dentures, the chemical residues of CHX may result in some degree of toxicity to the denture-bearing mucosa after long-term daily immersion. Highlights: Chemical residues of denture cleansers remain impregnated into acrylic bases even after rinsing the disinfected dentures. 2%CHX impregnated into acrylic resin was severely cytotoxic to human gingival fibroblasts after long-term immersion. Residues of 2%CHX may result in toxicity to the denture-bearing mucosa after long periods of daily overnight denture-soaking.