Quantification of methylation efficiency at a specific N6-methyladenosine position in rRNA by using BNA probes. Issue 69 (10th August 2018)

• Record Type: Journal Article
• Authors: Oshima, Takuya; Ishiguro, Kensuke; Suzuki, Tsutomu; Kawahara, Yukio
• Is Part Of: Chemical communications. Volume 54:Issue 69(2018)
• Page Start: 9627
• Page End: 9630
• Publication Date: 2018-08-10
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc03713b
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Probes containing bridged nucleic acids (BNA) enable us to quantify methylation efficiency at m 6 A sites in rRNA with high accuracy. Abstract : We found that insertion of artificial nucleic acid analogs, such as bridged nucleic acid (BNA), into DNA probes increases the difference in melting temperature between N 6 -methyladenosine (m 6 A)-containing RNA and unmethylated RNA. By applying this principle, we quantified methylation efficiency at m 6 A sites in E. coli 23S rRNA with high accuracy.