Conjugation of a GM3 lactone mimetic on carbon nanotubes enhances the related inhibition of melanoma-associated metastatic events. Issue 33 (9th August 2018)

• Record Type: Journal Article
• Authors: Arosio, Paolo; Comito, Giuseppina; Orsini, Francesco; Lascialfari, Alessandro; Chiarugi, Paola; Ménard-Moyon, Cécilia; Nativi, Cristina; Richichi, Barbara
• Is Part Of: Organic & biomolecular chemistry. Volume 16:Issue 33(2018)
• Page Start: 6086
• Page End: 6095
• Publication Date: 2018-08-09
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c8ob01817k
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Carbon nanotubes conjugated to a mimetic of a melanoma-associated antigen interfere with adhesion, motility, and invasiveness of human melanoma cells. Abstract : GM3-ganglioside is known to be involved in melanoma proliferation. In order to modulate metastatic-related events, we have functionalized multi-walled carbon nanotubes (MWCNTs) with multiple copies of a GM3-lactone mimetic. The MWCNTs proved to guarantee the appropriate spatial arrangement of the mimetic allowing a stronger inhibition of migration and invasiveness of human melanoma (A375) cells compared to other multivalent constructs reported before. In addition, the effect of the multivalent tubular conjugate on the inhibition of specific tyrosine kinases, which are associated with the ganglioside complexes within the membrane domains, was demonstrated. Finally, the short-term fate of the conjugate was assessed, for the first time, by means of the 1 H NMR relaxometry technique by exploiting the signal arising from the CNTs.