Incorporation of STED technique into single-molecule spectroscopy to break the concentration limit of diffusing molecules in single-molecule detection. Issue 69 (13th August 2018)

• Record Type: Journal Article
• Authors: Kim, Namdoo; Kwon, Jiwoong; Lim, Youngbin; Kang, Jooyoun; Bae, Sohyeon; Kim, Seong Keun
• Is Part Of: Chemical communications. Volume 54:Issue 69(2018)
• Page Start: 9667
• Page End: 9670
• Publication Date: 2018-08-13
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc05726e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Incorporation of STED into ALEX-FRET increases the concentration limit of single-molecule detection by 100-fold to 5 nM. Abstract : By incorporating STED (stimulated emission depletion) nanoscopy into single-molecule spectroscopy, we demonstrate that the concentration limit imposed by optical diffraction can be overcome in diffusion-based single-molecule measurement. We showed that single-molecule detection is feasible at a concentration of 5 nM, which is 100-times higher than the limit of conventional single-molecule measurements.