ExciTides: NTP-derived probes for monitoring pyrophosphatase activity based on excimer-to-monomer transitions. Issue 70 (14th August 2018)

• Record Type: Journal Article
• Authors: Wanat, Przemyslaw; Kasprzyk, Renata; Kopcial, Michal; Sikorski, Pawel J.; Strzelecka, Dominika; Jemielity, Jacek; Kowalska, Joanna
• Is Part Of: Chemical communications. Volume 54:Issue 70(2018)
• Page Start: 9773
• Page End: 9776
• Publication Date: 2018-08-14
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc04968h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We describe a new type of mononucleotide-derived fluorescent probe designed for monitoring pyrophosphatase activity based on excimer-to-monomer transitions. Abstract : We describe a new type of nucleotide-derived fluorescent probe designed for monitoring pyrophosphatase activity based on excimer-to-monomer transitions, called ExciTide. The nucleotides were designed with two self-interacting dye moieties and synthesised using copper-catalysed azide–alkyne cycloaddition click chemistry. We applied these probes for enzyme activity monitoring and inhibitor evaluation. Some of the probes permeated into living cells, yielding interesting prospects for future applications.