Effect of chitosan nanoparticles on the inhibition of Candida spp. biofilm on denture base surface. (October 2018)

• Record Type: Journal Article
• Authors: Gondim, Brenna Louise Cavalcanti; Castellano, Lúcio Roberto Cançado; de Castro, Ricardo Dias; Machado, Giovanna; Carlo, Hugo Lemes; Valença, Ana Maria Gondim; de Carvalho, Fabíola Galbiatti
• Is Part Of: Archives of oral biology. Volume 94(2018)
• Page Start: 99
• Page End: 107
• Publication Date: 2018-10
• Subjects: Chitosan -- Candida -- Biofilms -- Dental prosthesis -- Nanoparticles; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2018.07.004
• Languages: English
• ISSNs: 0003-9969

Abstract

Graphical abstract: Highlights: First investigation of chitosan nanoparticle (ChNPs) as daily denture disinfection. ChNPs inhibited the initial adherence and mature biofilm of C. albicans . After ChNPs exposure, the less virulent fungal form was predominant. ChNPs reduced the CFU Candida spp. on acrylic resin after 5 days biofilm. ChNPs caused minimal physical changes to the acrylic resin surface. Abstract: Objectives: Chitosan nanoparticles (ChNPs) have antifungal effects, however there is a lack of information about the effects of ChNPs against Candida biofilm on denture base surface. This study investigated the ChNPs effect against C. albicans biofilm adhesion and formation, and against Candida spp. biofilm on heat-cured acrylic resin. Design: The ChNPs were synthetized (3800 μg/mL) and characterized by infra-red spectrophotometry and transmission electron microscopy. The minimum inhibitory/fungicidal concentrations (MIC/MFC) against Candida spp. were determined. The time-kill assay and changes on C. albicans micromorphology were evaluated. The % inhibition of ChNPs on C. albicans biofilm formation and reduction were investigated using 1 min and 8 h exposure. Candida biofilm was developed on resin surfaces and ChNPs were applied every 8 h for 5 days. After, fungal cells were counted (CFU/mL) and the surface roughness (Ra) and vickers microhardness (HV) of resin were analysed. For all experiments, sodium hypochlorite (NaOCl) was used as control. Data were analyzed by ANOVA, Tukey and paired t -tests (α = 0.05). Results: The MIC80% of ChNPs was 30.1 μg/mL. ChNPs at 4 MIC showed complete inhibition in the time-kill assays. Blastoconidia cells were predominant after ChNPs application. The % inhibition ChNPs on C. albicans was proportional to its concentration, regardless of the exposure time. ChNPs decreased the CFU/mL of C andida spp. and showed lower alteration of HV and Ra values of resin surface compared to NaOCl. Conclusions: The ChNPs inhibited C. albicans biofilm, reduced Candida biofilm on resin and caused small changes in roughness and hardness of acrylic resin surface.