The role of granulocyte macrophage colony stimulating factor in hospitalized children with Mycoplasma pneumoniae pneumonia. Issue 10 (October 2018)

• Record Type: Journal Article
• Authors: Liu, Yang; Zhang, Xinxing; Wang, Yuqing; Zhu, Canhong; Fan, Mingyue; Dou, Xunwu; Hao, Chuangli; Yan, Yongdong; Ji, Wei; Gu, Guixiong; Lou, Jiangyan; Chen, Zhengrong
• Is Part Of: Journal of infection and chemotherapy. Volume 24:Issue 10(2018:Oct.)
• Page Start: 789
• Page End: 794
• Publication Date: 2018-10
• Subjects: Mycoplasma pneumoniae pneumonia -- Granulocyte macrophage colony stimulating factor -- Myeloperoxidase -- Normal human bronchial epithelium -- Children; MPP Mycoplasma pneumoniae pneumonia -- GM-CSF granulocyte macrophage colony stimulating factor -- MPO Myeloperoxidase -- CAP community acquired penumonia -- BALF bronchoalveolar lavage fluid -- NHBE normal human bronchial epithelial -- ELISA enzyme linked immunosorbent assay -- PCR polymerase chain reaction; Chemotherapy -- Periodicals; Infection -- Periodicals; Communicable diseases -- Chemotherapy -- Periodicals; 615.5805
• DOI: 10.1016/j.jiac.2018.06.003
• Languages: English
• ISSNs: 1341-321X

Abstract

Abstract: Background: Inappropriate inflammatory response in children with M. pneumoniae infection might be associated with disease severity. The role of Granulocyte macrophage colony stimulating factor (GM-CSF) in hospitalized children with Mycoplasma pneumoniae pneumonia (MPP) has not been fully discussed. Methods: Clinical and laboratory data of a total 40 children with MPP were collected. GM-CSF and myeloperoxidase (MPO) were detected by ELISAs. Meanwhile, normal human bronchial epithelium was infected by M. pneumoniae and neutrophils were stimulated by GM-CSF to explore GM-CSF and MPO release in supernatant, respectively. Results: Compared to control group, a significant increased percentage of neutrophils and decreased percentage of macrophages in bronchoalveolar lavage fluid of children with MPP was observed (P < 0.05). Children with MPP had significantly higher levels of GM-CSF (P = 0.0047) and MPO (P = 0.0002) in BALF compared to the controls. Level of GM-CSF in BALF was associated with duration of fever (r = 0.42, P = 0.007) and strongly correlated with level of MPO (r = 0.075, P = 0.0005). Levels of GM-CSF and MPO significantly decreased (both P < 0.05) after treatment. In vitro, M. pneumoniae induced GM-CSF expression in a time-dependent manner during a 72-h period (P < 0.05) and MPO secretion significantly increased by recombinant human GM-CSF stimulation at 24h (P < 0.05). Conclusion: GM-CSF could be induced by M. pneumoniae infection in vivo and vitro . Childen with high level GM-CSF had longer duration of fever. GM-CSF probably plays a vital role in neutrophil inflammation in M. pneumoniae infection.