Means to optimize protein expression in transgenic plants. (April 2015)

• Record Type: Journal Article
• Authors: Ullrich, Kristian K; Hiss, Manuel; Rensing, Stefan A
• Is Part Of: Current opinion in biotechnology. Volume 32(2015)
• Page Start: 61
• Page End: 67
• Publication Date: 2015-04
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2014.11.011
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: Multifactorial study designs result in improved prediction power. More focus should be placed on directional targeting to cell compartments. Previously neglected inside and outside factors are emerging. More emphasis on RNA structure to influence protein expression is in order. Abstract : The biotechnological production of proteins is currently achieved via expression systems derived from different lineages. In the past years transgenic plants have proven to be able to compete with bacteria or mammalian cell systems. Gene engineering approaches exist to raise yields by controlling mandatory processes in the course of biopharmaceutical protein production. Here we review and discuss the current status and recent improvements of parameters influencing recombinant protein production in transgenic plants. In particular, this review focuses on the so-called inside (mRNA sequence and structure) and outside factors (host and production system/conditions), which are adjustable and allow to optimize protein production via gene engineering.