F2-isoprostanes and fatty acids profile in early pregnancy complicated by pre-existing diabetes. (August 2018)

• Record Type: Journal Article
• Authors: Taschereau-Charron, Andréa; Bilodeau, Jean-François; Larose, Jessica; Greffard, Karine; Berthiaume, Line; Audibert, François; Fraser, William D.; Julien, Pierre; Rudkowska, Iwona
• Is Part Of: Prostaglandins, leukotrienes, and essential fatty acids. Volume 135(2018)
• Page Start: 115
• Page End: 120
• Publication Date: 2018-08
• Subjects: Hyperglycemia -- Oxidative stress -- Omega-3 fatty acids -- Delta-6-desaturase; AA arachidonic acid -- ALA alpha-linolenic acid -- BMI body mass index -- D5D delta-5-desaturase -- D6D delta-6-desaturase -- DGLA dihomo-gamma-linolenic -- DHA docosahexaenoic acid -- DPA docosapentaenoic acid -- EPA eicosapentaenoic acid -- ETA eicosatetraenoic acid -- F2-isoPs F2-isoprostanes -- FA fatty acids -- GC-FID gas chromatography coupled to a flame ionization detector -- HPLC-MS/MS high-performance liquid chromatography coupled to tandem mass spectrometry -- LA linoleic acid -- MIROS Maternal and Infant Research on Oxidative Stress -- MUFA monounsaturated fatty acids -- PUFA polyunsaturated fatty acids -- SFA saturated fatty acids; Lipids -- Periodicals; Unsaturated fatty acids -- Periodicals; Prostaglandins -- Periodicals; Leukotrienes -- Periodicals; Fatty Acids, Unsaturated -- Periodicals; Acides gras insaturés -- Périodiques; Prostaglandines -- Périodiques; Leucotriènes -- Périodiques; Lipides -- Périodiques; 612.01577
• DOI: 10.1016/j.plefa.2018.07.008
• Languages: English
• ISSNs: 0952-3278

Abstract

Highlights: Hyperglycemia in early pregnancy is associated with a specific F2 -isoprostane isomer and fatty acid profile in plasma. F2 -isoprostanes were elevated in diabetic pregnant women, particularly the 8-iso-15(R)-PGF2 α. Diabetic pregnant women had lesser n − 3 levels when compared to the control group, particularly EPA and DHA. The n − 3/n − 6 ratio and delta-6-desaturase (D6D) activity were lower in diabetic group compared to control group. Abstract: Background: Diabetes and pregnancy are both associated with oxidative stress, characterized by an increase of F2 -isoprostanes from the non-enzymatic oxidation of arachidonic acid, a n − 6 polyunsaturated fatty acid (PUFA). We hypothesized that pregnant women with pre-existing diabetes will be characterized by elevated levels of specific F2 -isoPs isomers and altered PUFA composition in plasma early pregnancy when compared to normoglycemic controls. Methods: Plasma samples from 23 women with uncomplicated pregnancies and 11 women with pre-existing diabetes in pregnancy were collected between 12 and 18 weeks of pregnancy (MIROS cohort). Six F2 -isoprostanes isomers were measured by high-performance liquid chromatography coupled to tandem mass spectrometry. Fatty acids concentrations in plasmatic phospholipids were measured by gas chromatography coupled to a flame ionization detector. Results: F2 -isoprostanes, specifically the 8-iso-15(R)-PGF2 α levels, were 67% higher in diabetic than normoglycemic pregnancies ( p = 0.026). The total n − 6 PUFA and arachidonic acid level did not differ between study groups. In contrast, total n − 3 level was 32% lower in diabetic pregnancies than in controls ( p = 0.002); EPA(20:5) and DHA(22:6) being specifically reduced ( p = 0.035 and p = 0.003 respectively). Delta-6-desaturase (D6D) activity index, calculated using fatty acid ratios, was 9% lower in pre-existing diabetes than in controls ( p = 0.042). Conclusions: Pre-existing diabetes in early pregnancy displays a distinctive F2 -isoprostanes profile when compared to other pathologies of pregnancy, such as preeclampsia, as previously assessed in the same cohort.