The supramolecular structure of LPS–chitosan complexes of varied composition in relation to their biological activity. (5th June 2015) - Davydova, V.N.
Highlights: Complexes between LPS from Escherihia coli ( E -LPS) and Yersinia pseudotuberculosis ( Y -LPS) and chitosan (Ch) were prepared. E -LPS–Ch complexes had different supramolecular structure. Complexes of Y -LPS–Ch had similar positive ζ -potential regardless of Ch content. Activity of E -LPS in complex with Ch decreased significantly in contrast to Y -LPS. Abstract: The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 ( E -LPS) and Yersinia pseudotuberculosis 1B 598 ( Y -LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ -potential distribution assay and atomic force microscopy. The alteration of ζ -potential of E -LPS–Ch complexes from negative to positive values depending on Ch content was detected. The Y -LPS–Ch complexes had similar positive ζ -potentials regardless of Ch content. The transformation of the supramolecular structure of E -LPS after binding with to Ch was revealed. Screening of E -LPS and Y -LPS particles by Ch in the complexes with high polycation was detected. The ability of LPS–Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied. A significant decrease in activity complexes compared to that of the initial LPS was observed only for E -LPS–Ch complexes.