Engineering human renal epithelial cells for transplantation in regenerative medicine. (October 2017)

• Record Type: Journal Article
• Authors: Manzoli, Vita; Colter, David C.; Dhanaraj, Sridevi; Fornoni, Alessia; Ricordi, Camillo; Pileggi, Antonello; Tomei, Alice A.
• Is Part Of: Medical engineering & physics. Volume 48(2017)
• Page Start: 3
• Page End: 13
• Publication Date: 2017-10
• Subjects: Three-dimensional culture -- Cell encapsulation -- Conformal coating -- Hydrogel -- Gene therapy -- Trophic factors; 2D Two-dimensional -- 3D Three-dimensional -- ALG alginate -- CCE conformal coating encapsulation -- DTT dithiothreitol -- dVS divinyl sulfone -- EGF epidermal growth factor -- FGFb basic fibroblast growth factor -- GFP green fluorescent protein -- GM-CSF granulocyte macrophage colony-stimulating factor -- HBSS Hanks' balanced salt solution -- HGF hepatocyte growth factor -- hRECs Human Renal Epithelial Cells -- MCP-1 monocyte chemotactic protein-1 (CCL2) -- MMP-2 matrix metalloproteinases-2 -- MOI multiplicity of infection -- MTT 3-(4, 5-Dimethylthiazol-2-yl)−2, 5-diphenyltetrazolium bromide -- PAI-1 plasminogen activator inhibitor-1 -- PBS phosphate-buffered saline -- PEG polyethylene glycol -- pHEMA Poly(2-hydroxyethyl methacrylate) -- PPG polypropylene glycol -- RPM revolutions per minute -- SCF stem cell factor -- TEA triethanolamine -- TGFα transforming growth factor alpha -- TIMP tissue inhibitor of metalloproteinase -- UP-MVG ultrapure medium viscosity (> 200 mPas) sodium alginate where minimum 60% of the monomer units are guluronate -- VCAM-1 vascular cell adhesion molecule-1 -- VEGF vascular endothelial growth factor; Biomedical engineering -- Periodicals; Biomedical Engineering -- Periodicals; Physics -- Periodicals; Génie biomédical -- Périodiques; Biomedical engineering; Electronic journals; Periodicals; 610.28
• DOI: 10.1016/j.medengphy.2017.03.009
• Languages: English
• ISSNs: 1350-4533

Abstract

Highlights: 3D culture of hRECs preserves long-term cell viability and trophic factor secretion. Genetically engineered hRECs retain long-term cell viability and secretome. Conformal coating of hRECs improves long-term cell viability and secretome. Abstract: Cellular transplantation may treat several human diseases by replacing damaged cells and/or providing a local source of trophic factors promoting regeneration. We utilized human renal epithelial cells (hRECs) isolated from cadaveric donors as a cell model. For efficacious implementation of hRECs for treatment of kidney diseases, we evaluated a novel encapsulation strategy for immunoisolation of hRECs and lentiviral transduction of the Green Fluorescent Protein (GFP) as model gene for genetic engineering of hRECs to secrete desired trophic factors. In specific, we determined whether encapsulation through conformal coating and/or GFP transduction of hRECs allowed preservation of cell viability and of their trophic factor secretion. To that end, we optimized cultures of hRECs and showed that aggregation in three-dimensional spheroids significantly preserved cell viability, proliferation, and trophic factor secretion. We also showed that both wild type and GFP-engineered hRECs could be efficiently encapsulated within conformal hydrogel coatings through our fluid dynamic platform and that this resulted in further improvement of cell viability and trophic factors secretion. Our findings may lay the groundwork for future therapeutics based on transplantation of genetically engineered human primary cells for treatment of diseases affecting kidneys and potentially other tissues.