Gut Lymphocyte Phenotype Changes After Parenteral Nutrition and Neuropeptide Administration. Issue 1 (July 2015)

• Record Type: Journal Article
• Authors: Jonker, Mark A.; Heneghan, Aaron F.; Fechner, John H.; Pierre, Joseph F.; Sano, Yoshifumi; Lan, Jinggang; Kudsk, Kenneth A.
• Is Part Of: Annals of surgery. Volume 262:Issue 1(2015:Jul.)
• Publication Date: 2015-07
• Subjects: bombesin -- lymphocyte phenotype -- mucosal immunity -- parenteral nutrition -- T regulatory cells; Surgery -- Periodicals; 617.005
• DOI: 10.1097/SLA.0000000000000878
• Languages: English
• ISSNs: 0003-4932

Abstract

Abstract : Objective: To define gut-associated lymphoid tissue (GALT) phenotype changes with parenteral nutrition (PN) and PN with bombesin (BBS). Background: PN reduces respiratory tract (RT) and GALT Peyer patch and lamina propria lymphocytes, lowers gut and RT immunoglobulin A (IgA) levels, and destroys established RT antiviral and antibacterial immunity. BBS, an enteric nervous system neuropeptide, reverses PN-induced IgA and RT immune defects. Methods: Experiment 1: Intravenously cannulated ICR mice received chow, PN, or PN + BBS injections for 5 days. LSR-II flow cytometer analyzed Peyer patches and lamina propria isolated lymphocytes for homing phenotypes (L-selectin + and LPAM-1 + ) and state of activation (CD25 +, CD44 + ) in T (CD3 + )-cell subsets (CD4 + and CD8 + ) along with homing phenotype (L-selectin + and LPAM-1 + ) in naive B (IgD + ) and antigen-activated (IgD − or IgM + ) B (CD45R/B220 + ) cells. Experiment 2: Following the initial experiment 1 protocol, lamina propria T regulatory cell phenotype was evaluated by Foxp3 expression. Results: Experiment 1: PN significantly reduced lamina propria (1) CD4 + CD25 + (activated) and (2) CD4 + CD25 + LPAM-1 + (activated cells homed to the lamina propria) T cells, whereas PN-BBS assimilated chow levels. PN significantly reduced lamina propria (1) IgD + (naive), (2) IgD − LPAM + (antigen-activated homed to the lamina propria) and CD44 + memory B cells, whereas PN-BBS assimilated chow levels. Experiment 2: PN significantly reduced lamina propria CD4 + CD25 + Foxp3 + T regulatory cells compared with chow-fed mice, whereas PN + BBS assimilated chow levels. Conclusions: PN reduces lamina propria activated and T regulatory cells and also naive and memory B cells. BBS addition to PN maintains these cell phenotypes, demonstrating the intimate involvement of the enteric nervous system in mucosal immunity. Abstract : Parenteral nutrition depresses mucosal immunity and lowers immunoglobulin A levels in the gut and the respiratory tract, whereas the neuropeptide bombesin reverses these changes. Lymphocyte phenotypes in the gut were analyzed by flow cytometry in mice fed enterally, parenterally, and parenterally with bombesin injections. Bombesin prevented lymphocyte phenotype changes seen with parenteral nutrition.