Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK. (August 2018)

• Record Type: Journal Article
• Authors: Mashima, Izumi; Djais, Ariadna A.; Haase, Elaine M.; Scannapieco, Frank A.; Otomo, Maiko; Saitoh, Masato; Nakazawa, Futoshi
• Is Part Of: Anaerobe. Volume 52(2018)
• Page Start: 79
• Page End: 82
• Publication Date: 2018-08
• Subjects: Veillonella -- Veillonella infantium -- dnaK gene -- Species-specific primer -- Japanese children -- Tongue biofilm; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2018.06.004
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: Recently, Veillonella infantium was isolated from tongue biofilm of a Thai child and established as a novel Veillonella species. In this study, a species-specific primer was designed to identify V. infantium on the basis of the sequence of the 70 kDa heat shock protein ( dnaK ) gene of Veillonella infantium JCM 31738 T (= TSD-88 T ). The primer pair generated a specific PCR (Polymerase Chain Reaction) product specific for V. infantium, but not for other oral Veillonella species. This specific primer pair could detect dnaK even from 1 pg of genomic DNA extracted from the V. infantium type strain. To validate the primer pair, a number of strains of Veillonella species were isolated from tongue biofilm of 3 Japanese children, DNA was isolated from each strain, and PCR was performed using species-specific primers. All oral Veillonella species except V. infantium were identified by one-step PCR method reported previously. Four kinds of Veillonella species were detected in these subjects. V. rogosae was detected in all subjects and the most predominant species with an average prevalence of 82%. However, V. infantium was detected in 2 of 3 subjects and it was the second most predominant species of oral Veillonella detected in these subjects with an average prevalence of 9.4%. V. infantium appears to coexist with other oral Veillonella species in tongue biofilm. This species-specific primer pair established in this study could be useful to detect V. infantium and support the study of Veillonella for oral health in the future. Highlights: Oral Veillonella have been isolated frequently from various sites of oral diseases. V. infantium ( V. i ) was recently established as novel species of genus Veillonella . Species-specific primer pair of V. i was designed based on the dnaK gene sequence. This primer pair detected V. i in tongue biofilm of Japanese children specifically. This species-specific primer pair could be useful for future Veillonella study.