Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometry. Issue 22 (6th February 2015)

• Record Type: Journal Article
• Authors: Fujita, Hideaki; Esaki, Tsuyoshi; Masujima, Tsutomu; Hotta, Akitsu; Kim, Soo Hyeon; Noji, Hiroyuki; Watanabe, Tomonobu M.
• Is Part Of: RSC advances. Volume 5:Issue 22(2015)
• Page Start: 16968
• Page End: 16971
• Publication Date: 2015-02-06
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c4ra12021c
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : By trapping individual single cells in a micro-well, molecules secreted by a single cell can be analyzed using mass spectrometry. Abstract : Secretomics, the comprehensive study of cell releasates, offers a non-invasive approach to understanding cell heterogeneity. We here propose single cell small molecule secretomics using micro-droplet arrays and mass spectrometry as a new repertoire of omics technologies. The present method revealed the heterogeneity of secreted small molecules from individual single cells without the use of any invasive processes.