Triterpenoid saponins from Clematis chinensis and their inhibitory activities on NO production. (September 2017)

• Record Type: Journal Article
• Authors: Fu, Qiang; Qiu, Lu; Yuan, Hai-Mei; Du, Yu-Feng; Cheng, Xiao-Ling; Tang, Ting-Ting; Wang, Ping; Xu, Ling; Liang, Jun-Ming; Yang, Long-Fei; Zou, Liang; Xu, Shu-Yun
• Is Part Of: Phytochemistry letters. Volume 21(2017:Sep.)
• Page Start: 206
• Page End: 210
• Publication Date: 2017-09
• Subjects: Triterpenoid saponins -- Clematis chinensis -- Anti-inflammatory activity -- Nitric oxide production; Botanical chemistry -- Periodicals; Chimie végétale -- Périodiques; 572.205
• DOI: 10.1016/j.phytol.2017.07.004
• Languages: English
• ISSNs: 1874-3900

Abstract

Graphical abstract: Highlights: 21α-hydroxyoleanolic acid type saponins from the roots and rhizomes of Clematis chinensis were studied. Four new compounds were isolated. Their structures were elucidated on the basis of spectroscopic evidence. Inhibitory effects against NO production in LPS-induced RAW 246.7 macrophages were investigated. Abstract: Four new triterpenoid saponins, clematochinenoside H–K (1 –4 ), and five known structures (5 –9 ), were isolated from the roots and rhizomes of Clematis chinensis . Their structures were elucidated on the basis of spectroscopic evidence and hydrolysis products. All isolates were evaluated for inhibitory effects against nitric oxide (NO) production in LPS-induced RAW 246.7 macrophages. Monodesmosidic saponins (1 –3, 5, and6 ) with a free carboxylic acid function at C-28 exhibited potent inhibitory activities with IC50 values in the range of 12.9–32.3 μM, where as bisdesmosidic saponin (4, and7–9 ) showed modest inhibitory effects with the inhibition ratios (%) from 39.9 to 59.0 at 50 μM. In addition, the hydroxyl group at C-21 showed negative effect on the NO production inhibitory activity.