Generation 3 PAMAM dendrimer TAMRA conjugates containing precise dye/dendrimer ratios. (September 2015)

• Record Type: Journal Article
• Authors: Manono, Janet; Dougherty, Casey A.; Jones, Kirsten; DeMuth, Joshua; Holl, Mark M.Banaszak; DiMaggio, Stassi
• Is Part Of: Materials today communications. Volume 4(2015)
• Page Start: 86
• Page End: 92
• Publication Date: 2015-09
• Subjects: PAMAM polyamidoaminedendrimer -- TAMRA 5-carboxytetramethylrhodamine; Poly(amidoamine) dendrimer -- PAMAM -- Fluorescent dye -- rp-HPLC; Materials science -- Periodicals; 620.11
• DOI: 10.1016/j.mtcomm.2015.06.006
• Languages: English
• ISSNs: 2352-4928

Abstract

Graphical abstract: Abstract: The synthesis, isolation, and characterization of generation 3 poly(amidoamine) (G3 PAMAM) dendrimer containing precise ratios of 5-carboxytetramethylrhodamine succinimidyl ester (TAMRA) dye ( n = 1–3) per polymer particle are reported. Stochastic conjugation of TAMRA dye to the dendrimer was followed by separation into precise dye-polymer ratios using rp-HPLC. The isolated materials were characterized by rp-UPLC, MALDI-TOF-MS, and 1 H NMR spectroscopy, UV–vis, and fluorescence spectroscopies.