Chemodiversity of Clausena excavata (Rutaceae) and related species: Coumarins and carbazoles. (October 2018) - Wongthet, Natthawadi
Abstract: Methanolic crude extracts obtained from leaves, stem bark and root bark of 57 accessions of Clausena excavata, C . harmandiana, of both varieties of C . wallichii, as well as of three unidentified Clausena samples, were analysed for the presence of coumarins and carbazoles. All accessions were collected from different locations all over Thailand. HPLC coupled with UV-DAD was applied for phytochemical profiling. Structures of known pyrano-coumarins1 –4 and of coumarins5 –8 bearing an O -geranyl moiety, as well as of carbazoles9 –12 were confirmed by NMR and MS spectroscopy, respectively. Observed chemical diversification in the studied samples is discussed in relation to geographic distribution and with respect to organ-specific accumulation. Chemotaxonomic significance is briefly addressed. Graphical abstract: Highlights: 57 Accessions of three Clausena species were studied phytochemically. Eight coumarins and four carbazoles were isolated. Organ specific accumulation of compounds1 –12 was studied in all accessions. Chemical diversity in relation to geographic distribution patterns is discussed.