Estimation of G-quartet-forming guanines in parallel-type G-quadruplexes by optical spectroscopy measurements of their single-nucleobase substitution sequences. Issue 17 (9th August 2018)

• Record Type: Journal Article
• Authors: Maruyama, Ryo; Makino, Kurumi; Yoshitomi, Toru; Yui, Hiroharu; Furusho, Hitoshi; Yoshimoto, Keitaro
• Is Part Of: Analyst. Volume 143:Issue 17(2018)
• Page Start: 4022
• Page End: 4026
• Publication Date: 2018-08-09
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an01131a
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Here, we established a new estimation method for G-quartet-forming guanines in parallel-type G-quadruplexes using measurements of circular dichroism and thermal stability. Abstract : Since much attention has been paid to in vivo biological functions of G-quadruplexes, structural analyses of G-quadruplexes are essential for understanding their functional mechanisms. Here, we established a simple optical-spectroscopy-based method for the estimation of G-quartet-forming guanines in parallel-type G-quadruplexes using measurements of circular dichroism and the thermal melting temperature.