A guanidyl-functionalized TiO2 nanoparticle-anchored graphene nanohybrid for enhanced capture of phosphopeptides. Issue 51 (20th August 2018)

• Record Type: Journal Article
• Authors: Liu, Hailong; Lian, Bin
• Is Part Of: RSC advances. Volume 8:Issue 51(2018)
• Page Start: 29476
• Page End: 29481
• Publication Date: 2018-08-20
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra05006f
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A novel TiO2 -based MOAC hybrid nanomaterial was successfully synthesized and applied as a biofunctional adsorbent for selective enrichment of trace phosphopeptides. Abstract : TiO2 -based MOAC (metal oxide affinity chromatography) nanomaterials are regarded as one of the most promising materials for phosphopeptide enrichment. However, the serious non-specific adsorption of acidic peptides and the limited chemisorption performance to phosphopeptides will greatly reduce the enrichment efficiency. To overcome the above problems, a novel TiO2 hybrid material with guanidyl-functionalized TiO2 nanoparticles (GF-TiO2 ) anchored on the surface of a graphene oxide (GO) platform (denoted as GF-TiO2 –GO) is successfully synthesized and applied as a biofunctional adsorbent for selective enrichment of trace phosphopeptides. Due to the improved selectivity to phosphopeptides and larger loading capacity, the novel GF-TiO2 –GO nanohybrids exhibited higher selectivity toward phosphopeptides and a lower detection limit even when the concentration of β-casein was decreased to only 1 × 10 −11 M. The selective enrichment test toward phosphopeptides from the tryptic digests of nonfat milk and human serum further validated that the GF-TiO2 –GO nanohybrids were capable of selectively capturing global phosphopeptides from complicated biological samples.