Artificial reproduction protocol, from spawning to metamorphosis, through noninvasive methods in Patella caerulea Linnaeus, 1758. (19th August 2018)

• Record Type: Journal Article
• Authors: Ferranti, Maria Paola; Monteggia, Davide; Asnaghi, Valentina; Chiantore, Mariachiara
• Is Part Of: Aquaculture research. Volume 49:Number 10(2018)
• Page Start: 3386
• Page End: 3391
• Publication Date: 2018-08-19
• Subjects: artificial reproduction -- limpet aquaculture -- Patella caerulea -- spawning induction; Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.13802
• Languages: English
• ISSNs: 1355-557X

Abstract

Abstract: Controlled reproduction is a requirement for developing effective mollusc cultivation for commercial or restoration purposes. In this study, a protocol for spawning induction using noninvasive methods in limpets was developed, using the common Mediterranean species, Patella caerulea Linnaeus, 1758. Six nonlethal spawning induction treatments were tested: three chemical (two concentrations of H2 O2 and KCl) and three physical (bubbling, warm and cold thermal shock). All treatments, except thermal shocks, induced the spawning of fertile gametes. Bubbling resulted the best treatment in providing spawning response, being the easiest and least invasive method tested. After eggs fertilization, larval development was followed until metamorphosis, testing fed and unfed conditions. Settlement took place after 7 days. The developed protocol represents a benchmark for further application to other limpets, for aquaculture or repopulation.