Structural characterization and antioxidant activity of a heteropolysaccharide from Ganoderma capense. (5th May 2015)

• Record Type: Journal Article
• Authors: Yi, Pan; Li, Naisheng; Wan, Jian-Bo; Zhang, Dezhi; Li, Mingya; Yan, Chunyan
• Is Part Of: Carbohydrate polymers. Volume 121(2015)
• Page Start: 183
• Page End: 189
• Publication Date: 2015-05-05
• Subjects: Ganoderma capense -- Polysaccharide -- Structural characterization -- Antioxidant activity.; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2014.11.034
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A novel polysaccharide (GCPB-3) was isolated from mycelia of Ganoderma capense (Lloyd) Teng. The relative molecular weight is 124 kDa and a specific optical rotation is [ α ] D 25 = +145°. GCPB-3 is a heteropolysaccharide with backbone consisting of β-l -Ara p and β-d -Xyl p, linked with 1→4 sugar bonds. GCPB-3 has a certain activity of DPPH- and hydroxy-radical scavenging. Abstract: In this work, crude polysaccharide extracts were obtained from mycelia of the edible fungus Ganoderma capense (Lloyd) Teng. After removal of proteins by the Sevage method, fractionation and purification by anion-exchange and gel-permeation chromatography, a polysaccharide (GCPB-3) was isolated. The relative molecular weight of GCPB-3 was 124 kDa determined by high performance gel permeation chromatography (HPGPC). The homogeneous polysaccharide was composed ofd -xylose andl -arabinose in the ratio of 1:1, and showed a specific optical rotation of [ α ] D 25 = +145°(c 1.0, H2 O). Its structural features were determined by monosaccharide analysis, partial acid hydrolysis, methylation analysis, periodic acid oxidation, gas chromatography-mass spectrometry (GC-MS), Fourier transform-infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy ( 1 H, 13 C, HMQC and HMBC). The results characterized GCPB-3 as a heteropolysaccharide with backbone consisting of β-l -Ara p and β-d -Xyl p, linked with 1→4 sugar bonds. Interestingly, GCPB-3 showed some DPPH- and hydroxy-radical scavenging activities.