Hypoglycemic activity and potential mechanism of a polysaccharide from the loach in streptozotocin-induced diabetic mice. (5th May 2015)

• Record Type: Journal Article
• Authors: Zhou, Jun; Yan, Junyan; Bai, Zhaoshuai; Li, Kaicheng; Huang, Kaixun
• Is Part Of: Carbohydrate polymers. Volume 121(2015)
• Page Start: 199
• Page End: 206
• Publication Date: 2015-05-05
• Subjects: ALT alanine aminotransferase -- ANOVA analysis of variance -- AST aspartate aminotransferase -- BSA bovine serum albumin -- BUN blood urea nitrogen -- BW body weight -- CHD coronary heart disease -- FBG fasting blood glucose -- FT-IR Fourier transform infrared -- GC gas chromatography -- GPx glutathione peroxidase -- HDL-C high density lipoprotein-cholesterol -- IL-6 interleukin-6 -- LDL-C low density lipoprotein-cholesterol -- MAP Misgurnus anguillicaudatus polysaccharide -- MCP-1 monocyte chemoattractant protein-1 -- MDA malondialdehyde -- M-MLV molony murine leukemia virus -- PBS phosphate buffered saline -- PEPCK phosphoenolpyruvate carboxyl kinase -- RT-PCR reverse transcription-polymerase chain reaction -- SEM standard error of the mean -- SOD superoxide dismutase -- STZ streptozotocin -- TBARS thiobarbituric acid reactive substances -- TC total cholesterol -- TG triglycerides -- TNF-α tumor necrosis factor-alpha; Misgurnus anguillicaudatus polysaccharide -- Diabetes -- Insulin -- Dyslipidemia -- Oxidative stress -- Inflammation; Streptozotocin (PubChem CID: 29327) -- Glucose (PubChem CID: 5793) -- Metformin (PubChem CID: 4091) -- Cholesterol (PubChem CID: 5997) -- Malondialdehyde (PubChem CID: 10964) -- Glutathione (PubChem CID: 124886); Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2014.12.037
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: MAP improves hyperglycemia, hyperlipemia and vascular inflammation in diabetic mice. MAP improves vascular and pancreatic oxidative stress in diabetic mice. MAP causes increases in serum and pancreatic insulin contents in diabetic mice. MAP improves hepatic gluconeogenesis and glycogen synthesis in diabetic mice. MAP may be a potential therapeutic option for type 1 diabetes. Abstract: The present study was designed to investigate the hypoglycemic activity and the potential mechanisms of Misgurnus anguillicaudatus polysaccharide (MAP) in streptozotocin-induced diabetic mice. MAP oral administration significantly decreased the blood levels of glucose, TC, TG, LDL-C, and increased the blood levels of HDL-C and insulin in diabetic mice, concurrent with increases in body weights and pancreatic insulin contents. Moreover, MAP reversed the increased mRNA expressions of PEPCK and the reduced glycogen contents in the liver of diabetic mice. Concurrently, MAP exhibited potent anti-inflammatory and anti-oxidative activities, as evidenced by the decreased blood levels of TNF-α, IL-6, monocyte chemoattractant protein-1, MDA, and also the elevated SOD and GPx activities in the serum of diabetic mice. Furthermore, MAP also significantly improved the blood markers of the impaired liver function and renal function in diabetic mice. Altogether, these results suggest that MAP may be a potential therapeutic option for type 1 diabetes.