Mast Cells Regulate CD4+ T Cell Differentiation in Absence of Antigen Presentation. (July 2018)

• Record Type: Journal Article
• Authors: Biefer, Hector Rodriguez Cetina; Heinbokel, Timm; Uehara, Hirofumi; Camacho, Virginia; Koduru, Suresh; El Fatimy, Rachid; Ghiran, Ionita; Trachtenberg, Alexander J; de la Fuente, Miguel A; Azuma, Haruhito; Akbari, Omid; Vasudevan, Anju; Tullius, Stefan G; Elkhal, Abdallah
• Is Part Of: Transplantation. Volume 102(2018)Supplement 7S-1
• Publication Date: 2018-07
• Subjects: Transplantation of organs, tissues, etc -- Periodicals; Transplantation immunology -- Periodicals; 617.95
• DOI: 10.1097/01.tp.0000542984.70770.b1
• Languages: English
• ISSNs: 0041-1337

Abstract

Abstract : Given their unique capacity for antigen uptake, processing, and presentation, antigen presenting cells (APCs) are critical for initiating and regulating innate and adaptive immune responses. Using bone marrow-derived mast cells and mast cell (MC) deficient mice, our in vivo and in vitro findings indicate that following NAD + administration MCs, exclusively, promote CD4 + T-cell differentiation, both in absence of antigen and independently of major APCs. Moreover, we found that MCs mediated CD4 + T-cell differentiation independently of MHC-II and TCR signaling machinery. Although treatment with NAD + resulted in a decreased MHC-II expression on CD11c + cells, MC-mediated CD4 + T-cell differentiation rendered mice resistant to lethal doses of Listeria monocytogenes. Collectively, our study unravels a novel cellular and molecular pathway that regulates innate and adaptive immunity via MCs and may pave the way for novel therapeutic approaches in the context of immunodeficiencies and antimicrobial resistance.