ALKBH5-induced demethylation of mono- and dimethylated adenosine. Issue 62 (16th July 2018)

• Record Type: Journal Article
• Authors: Ensfelder, Timm T.; Kurz, Matthias Q.; Iwan, Katharina; Geiger, Simon; Matheisl, Sarah; Müller, Markus; Beckmann, Roland; Carell, Thomas
• Is Part Of: Chemical communications. Volume 54:Issue 62(2018)
• Page Start: 8591
• Page End: 8593
• Publication Date: 2018-07-16
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc03980a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : ALKBH5 is able to accept m 6 2 A as a substrate and demethylates it to m 6 A and A. Abstract : RNA contains methylated A-base derivatives. A methylation to m 6 A and then demethylation regulate homeostasis in mRNA. It is assumed that m 6 A is mainly demethylated by the α-ketoglutarate dependent oxidase ALKBH5. Here we show that ALKBH5 also demethylates the dimethylated adenosine m 6 2 A, which is a non-canonical base present in ribosomal RNA.