A new fluorescent probe for quick and highly selective detection of hydrogen sulfide and its application in living cells. (25th July 2018)

• Record Type: Journal Article
• Authors: Ren, Yanshen; Zhang, Lulu; Zhou, Ziyan; Wang, Suilou; Xu, Yungen; Gu, Yueqing; Zha, Xiaoming
• Is Part Of: New journal of chemistry. Volume 42:Number 16(2018)
• Page Start: 13884
• Page End: 13888
• Publication Date: 2018-07-25
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c8nj02178c
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : Hydrogen sulfide (H2 S) is one of the endogenous regulators of many physiological processes. Abstract : Hydrogen sulfide (H2 S) is one of the endogenous regulators of many physiological processes.NIPY-OH based on an imidazo[1, 5-α]pyridine has a large Stokes shift, high photostability with green-emission, relatively high fluorescence quantum yield.NIPY-OH could serve as an excellent candidate for the design of fluorescent probes. However, the reported probes cannot sufficiently take advantage of the optical properties ofNIPY-OH due to the water-soluble problem. Herein, a new fluorescent probeNIPY-PBA, based onNIPY-OH, was designed for the detection of hydrogen sulfide. By rational design, compared with other probes, we successfully obtained the probe with higher sensitivity (limit of detection = 43 nM) and shorter response time (1.5 minutes) due to better water solubility.NIPY-PBA maintained the excellent optical properties of a 230-fold fluorescence enhancement with a large Stokes shift (208 nm) at the same time. Furthermore, NIPY-PBA was applied for the fluorescence imaging of H2 S in living cells with low cytotoxicity, indicatingNIPY-PBA has great potential to monitor H2 S in biological systems.