A novel FRET-based fluorescent probe for the selective detection of hydrogen sulfide (H2S) and its application for bioimaging. Issue 30 (20th July 2018)

• Record Type: Journal Article
• Authors: Zhao, Qi; Huo, Fangjun; Kang, Jin; Zhang, Yongbin; Yin, Caixia
• Is Part Of: Journal of materials chemistry. Volume 6:Issue 30(2018)
• Page Start: 4903
• Page End: 4908
• Publication Date: 2018-07-20
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c8tb01070f
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Herein, our group developed a fast response fluorescent probe (Flu-N3 ) for H2 S on the basis of the 7-amino-4-methylcoumarin and fluorescein FRET system with high sensitivity and selectivity and a low detection limit of 0.031 μM. Moreover, the probe was successfully applied to image exogenous and endogenous H2 S in living cells and nude mice. Abstract : Hydrogen sulfide (H2 S) is an endogenously produced gaseous signaling molecule with multiple functions in many physiological and pathological processes. Herein, our group developed a novel fluorescent probe (Flu-N3 ) for H2 S on the basis of the 7-amino-4-methylcoumarin and fluorescein FRET system. Adding H2 S to a solution ofFlu-N3 resulted in a clearly observable fluorescence enhancement (50-fold). This probe showed high sensitivity and selectivity towards H2 S over other reactive species with a detection limit of 0.031 μM and a fast response time (within 8 s). Moreover, Flu-N3 displayed little toxicity to HepG-2 cells and was successfully applied to image exogenous and endogenous H2 S in living cells and nude mice.