Fluorescence assay for alkaline phosphatase based on ATP hydrolysis-triggered dissociation of cerium coordination polymer nanoparticles. Issue 16 (16th July 2018)

• Record Type: Journal Article
• Authors: Chen, Chuanxia; Yuan, Qun; Ni, Pengjuan; Jiang, Yuanyuan; Zhao, Zhenlu; Lu, Yizhong
• Is Part Of: Analyst. Volume 143:Issue 16(2018)
• Page Start: 3821
• Page End: 3828
• Publication Date: 2018-07-16
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an00787j
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : We have developed a fluorescence assay for ALP based on ATP hydrolysis-triggered dissociation of coordination polymer nanoparticles. Abstract : Alkaline phosphatase (ALP) is a significant biomarker for diagnostics. Simple, selective and sensitive detection of ALP activity is thus of critical importance. In this study, an artful fluorescence assay for ALP is proposed based on adenosine triphosphate (ATP) hydrolysis-triggered disassociation and fluorescence quenching of cerium coordination polymer nanoparticles (CPNs). ATP, a recognized natural substrate of phosphatase, can serve as a superb "antenna" to sensitize the luminescence of Ce 3+ with the aid of tris(hydroxymethyl) aminomethane (Tris), forming Ce 3+ –ATP–Tris CPNs. In the presence of ALP, ATP will be catalytically converted into adenosine and inorganic orthophosphate, however neither of them can sensitize Ce 3+ in alkaline media. As a result, the obtained CPNs are disassociated, inducing the quenching of the fluorescence. On this basis, a straightforward fluorescence assay for ALP activity is rationally developed. The fluorescence quenching efficiency shows a linear relationship for ALP within the activity range from 0.1 to 10 mU mL −1 with a detection limit of 0.09 mU mL −1 under the optimal experimental conditions. Moreover, this facile yet effective fluorescence method featured simplicity, cost-effectiveness, high sensitivity and high selectivity and can be successfully utilized for the quantitative detection of ALP in human serum samples.