Discrimination of Cynanchum wilfordii and Cynanchum auriculatum by terahertz spectroscopic analysis. Issue 5 (12th February 2018)

• Record Type: Journal Article
• Authors: Ham, Woo Sik; Kim, Jinju; Park, Dae Joon; Ryu, Han‐Cheol; Jang, Young Pyo
• Is Part Of: Phytochemical analysis. Volume 29:Issue 5(2018)
• Page Start: 472
• Page End: 475
• Publication Date: 2018-02-12
• Subjects: Cynanchum auriculatum -- Cynanchum wilfordii -- discrimination -- terahertz time domain spectroscopy; Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2751
• Languages: English
• ISSNs: 0958-0344

Abstract

Abstract: Introduction: Precise identification of botanical origin of plant species is crucial for the quality control of herbal medicine. In Korea, the root part of Cynanchum auriculatum has been misused for C. wilfordii in the herbal drug market due to their morphological similarities. Currently, DNA analysis using the polymerase chain reaction (PCR) method is employed to discriminate between these species. Objective: In order to develop a new analytical tool for the rapid discrimination of C. wilfordii and C. auriculatum, terahertz (THz) spectroscopy was employed. Methodology: Authentic samples of C. wilfordii and C. auriculatum were provided from the National Institute and standardized pellets for each species were prepared to get optimum results with terahertz time‐domain spectroscopy (THz‐TDS) in frequency range 0.2–1.20 THz. Results: The C. wilfordii pellet showed longer time delay compare to the sample of C. auriculatum and this was due to the difference in permittivity. The pellet samples of C. wilfordii and C. auriculatum showed a permittivity difference of about 0.08 at 0.2–1.20 THz. Conclusion: The experimental results indicated that THz‐TDS analysis can be an effective and rapid method for the discrimination of C. wilfordii and C. auriculatum, and this application can be expanded for the discrimination of other similar herbal medicines. Abstract : Terahertz time‐domain spectroscopy (THz‐TDS) was employed for the discrimination of two similar Cynancum species. Cynancum wilfordii pellet showed longer time delay compare to the sample of C. auriculatum and this was due to the difference in the permittivity between two species, about 0.08 at 0.2–1.20 THz. THz‐TDS analysis can be an effective and rapid method for the discrimination of C. wilfordii and C. auriculatum .