Aging and nutrition induce tissue-specific changes on global DNA methylation status in rats. (September 2018)

• Record Type: Journal Article
• Authors: Guarasci, Francesco; D'Aquila, Patrizia; Mandalà, Maurizio; Garasto, Sabrina; Lattanzio, Fabrizia; Corsonello, Andrea; Passarino, Giuseppe; Bellizzi, Dina
• Is Part Of: Mechanisms of ageing and development. Volume 174(2018)
• Page Start: 47
• Page End: 54
• Publication Date: 2018-09
• Subjects: Global DNA methylation -- Aging -- Tissue specificity -- Nutrition -- Low-calorie diet -- Maternal pre-gestational/gestational diet; Aging -- Periodicals; Developmental biology -- Periodicals; Aging -- Periodicals; Developmental Biology -- Periodicals; Vieillissement -- Périodiques; Biologie du développement -- Périodiques; Aging; Developmental biology; Periodicals; 612.67
• DOI: 10.1016/j.mad.2018.02.001
• Languages: English
• ISSNs: 0047-6374

Abstract

Highlights: Hyper-methylation takes place during aging in all tissues except for liver. Low-calorie diet affects global DNA methylation according to rat's tissues and age. Maternal pre-gestational diet influences global DNA methylation in offspring. Abstract: A number of epigenetic studies have demonstrated that DNA methylation patterns exhibit a tissue specificity, but not much has been done to highlight the extent of this phenomenon. Moreover, it is unknown how external factors modulate the plasticity of the tissue specific epigenetic profile. We examined global DNA methylation profiles in tissues from rats of different age, fed with standard or low-calorie diet, and evaluated their association with aging and nutrition. Tissue-specific variations occur during aging with hyper-methylation taking place in all tissues except for liver. The expression of enzymes involved in methylation reactions (DNMTs and TETs) was consistent with the methylation patterns. Nutrition affects global DNA methylation status throughout lifespan. Interestingly, the differences among different tissues are magnified in 96 weeks old rats fed with low calorie diet. Moreover, the low-calorie diet appears to affect the offspring's epigenetic status more strongly if administered during the maternal pre-gestational period than the gestational and lactation time. Therefore, we propose that changes in the global DNA methylation status may represent an epigenetic mechanism by which age and nutrition intersect each other and, in turn, influence the aging plasticity.