Hypericin Loaded Liposomes for Anti‐Microbial Photodynamic Therapy of Gram‐Positive Bacteria. Issue 15 (27th February 2018)

• Record Type: Journal Article
• Authors: Plenagl, Nikola; Seitz, Benjamin Sebastian; Reddy Pinnapireddy, Shashank; Jedelská, Jarmila; Brüßler, Jana; Bakowsky, Udo
• Other Names: Wagner Patrick guestEditor.; Keusgen Michael guestEditor.; Doll Theodor guestEditor.; Wagner Torsten guestEditor.; Schöning Michael J. guestEditor.
• Is Part Of: Physica status solidi. Volume 215:Issue 15(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-02-27
• Subjects: antimicrobial -- hypericin -- liposomes -- photodynamic therapy; Solid state physics -- Periodicals; Solids -- Industrial applications -- Periodicals; 530.41
• DOI: 10.1002/pssa.201700837
• Languages: English
• ISSNs: 1862-6300

Abstract

Abstract : Due to its highly lipophilic nature, poor solubility in aqueous media, and poor bioavailability, the naturally occurring photosensitizer hypericin has a limited therapeutic value. Liposomal encapsulation is a promising solution to overcome these limitations. Use of liposomes as delivery vehicles for hypericin in antimicrobial photodynamic therapy (aPDT) is quite new. The aim of this work is therefore, to prepare various hypericin loaded liposomal formulations viz. DOPE/CHEMS/DPPC, DSPC/DPPC/DSPE‐PEG, and DPPC/DOTAP. The formulations are physicochemically characterized and tested for their binding affinity toward bacteria and for their photodynamic efficiency. All formulations achieve a 2.3–2.5 log reduction of Staphylococcus saprophyticus subsp . bovis and facilitate the binding and uptake of the photosensitizer through the bacterial cell wall. Abstract : Due to its highly lipophilic nature, the potential of hypericin as a photosensitizer is limited. In this study, liposomal encapsulation of the poorly soluble hypericin helped achieve a 2.5 log reduction in the bacterial viability upon irradiation at 589 nm using a diode laser.